TABLE OF CONTENTS
Liquid Scintillation Counting Volume 2
M.A. Crook, P. Johnson, and B. Scales, Editors
Proceedings of a Symposium
Organized by the Society for Analytical Chemistry
Brighton, England
PREFACE ....................................................................................................................... ix
I. BASIC THEORY OF THE CHEMISTRY AND PHYSICS OF
SCINTILLATORS
AND SCINTILLATION COUNTING
Liquid Scintillators
J B Birks and G C Poullis ................................................................................................. 1
Liquid Scintillation Counting as an Absolute Method
J A B Gibson ................................................................................................................. 23
Colour Quenching in Liquid Scintillation Coincidence Counters
F E L ten Haaf .............................................................................................................. 39
Application of Solid Scintillators in High Pressure Radio Column Chromatography
G B Seiswerda and H L Polach........................................................................................ 49
II. HISTORICAL DEVELOPMENTS AND MISCELLANEOUS PROBLEMS
A History of the Development of the Modern Liquid Scintillation Counter
E Rapkin ...................................................................................................................... 61
Questions Regarding the Occurrence of Unwanted Luminescence in
Liquid Scintillation Samples
B Scales ..................................................................................................................... 101
The Estimation of ATP, ADP, and AMP in Human Plasma using Luciferin/Luciferase
and a Scintillation Counter
P I Parkinson and E Medley.......................................................................................... 109
Semi-automatic Microtransfer and Cell for the Bioluminescence Assay of ATP and Reduced NAD with Scinitllation Counters
E Schram and H Roosens ........................................................................................... 115
III. METHODS OF SAMPLE PREPARATION OF INORGANIC MATERIALS
INCLUDING CERENKOV COUNTING
Methods of Sample Preparation of Inorganic Materials including Cerenkov Counting
A Dyer ....................................................................................................................... 121
Liquid Scintillation Counting of Low Levels of Carbon-14 for Radiocarbon Dating
R Burleigh .................................................................................................................. 139
Liquid Scintillation Counting of Calcium-45 in Biological Samples Containing Environmental Strontium-90
J Nolan ...................................................................................................................... 147
The Simultaneous Determination of Plutonium Alpha Activity and Plutonium-241 in Biological Materials by Gel Scintillation Counting
J D Eakins and A E Lally
Techniques for Counting Carbon-14 and Phosphorus-32 Labelled Samples of Polluted Natural Waters
E J C Curtis and I P Toms ........................................................................................... 167
Application of Cerenkov Technique to Continuous Measurement of Radioactive Isotopes Isolated by an Automatic Analytical Process
J Colomer, M Cousigne and G Metzger ......................................................................... 181
IV. METHODS OF SAMPLE PREPARATION OF ORGANIC MATERIALS
INCLUDING BIOLOGICAL SYSTEMS
Sample Preparation Techniques in Biochemistry with Particular Reference to Heterogeneous Systems
B W Fox ..................................................................................................................... 189
Liquid Scintillation Counting of Biological Macromolecules: Extraction from Aqueous Solution and from Glass Fibre Filters
P N Paus .................................................................................................................... 205
The Estimation of Small Quantities of Carbon-14 Labelled Adenine Nucleotides following their Separation by Ion Exchange Paper Chromatography
G C Carney ................................................................................................................ 213
Sample Preparation for Tritium Counting in the Application of the Digoxin Radioimmunoassay Technique to Lysed Blood
A P Phillips and C A Sambrook ..................................................................................... 217
Dynamics of Quenching by Plasma from Patients with Various Pathological Conditions
J Assailly, C Bader, J-L Funck-Brentano and D Pavel .................................................... 223
Factors Influencing the Detection of Incorporated H-3-Thymidine in Biological Material
W A Cope and J A Double ............................................................................................ 233
V. DATA PROCESSING
Acquisition and Handling of Liquid Scintillation Counting Data
J L Spratt ................................................................................................................... 245
Liquid Scintillation Counting of Biological Samples using External Standardization and Automatic Data Processing
P Johnson, P A Rising and T J Rising............................................................................. 267
Automatic Processing of Data from Liquid Scintillation Counters Illustrated by Drug Distribution Studies
H E Barber and G R Bourne ......................................................................................... 279
Determination of Absolute Radioactivity in Multi-Labelled Samples using External Standardization or Channels Ratio: A Fortran IV Program
P E Stanley ................................................................................................................ 285
Determination of Statistical Precision of Tritium dpm in Dual Labelled Samples with Variable Isotope Ratios and Quenching
J Assailly, C Bader, J-L Funck-Brentano and D Pavel .................................................... 293
Computer Data Handling for the Radiochemical Immunoassay of Insulin
G Ayrey and K L Evans ............................................................................................... 303
Experience in Off-Line Computer Processing of Liquid Scintillation Counting Data
J H Deterding ............................................................................................................. 313
SUBJECT INDEX ......................................................................................................... 325
INDEX TO CONTRIBUTORS ......................................................................................... 327